Berberine Suppresses EMT in Liver and Gastric Carcinoma Cells through Combination with TGFβR Regulating TGF-β/Smad Pathway
Table 1
Analyze BBR treatment by transcriptome sequencing assay.
(a) The target genes of BBR related to EMT were significantly upregulated
Gene_name
Trans_name
Description
Fold_change
_value
12 h/0 h
SMAD2
SMAD2-205
SMAD family member 2
6.7223
0.03633
SMAD2
SMAD2-207
SMAD family member 2
1.8956
0.0060
TAB2
TAB2-209
TGF-beta activated kinase 1 (MAP3K7) binding protein 2
1.5811
0.0015
SMAD6
SMAD6
SMAD family member 6
1.5090
0.0000
24 h/0 h
SMAD2
SMAD2-205
SMAD family member 2
6.9633
0.0215
TJAP1(ZO1)
TJAP1-201
Tight junction associated protein 1
2.2025
0.03990
CLDN7
CLDN7-201
Claudin 7
1.8872
0.00161
SMAD6
SMAD6
SMAD family member 6
1.5529
5.1607E-06
JMY
JMY-201
Junction mediating and regulatory protein, p53 cofactor
1.8839
7.4150E-09
SMAD2
SMAD2-207
SMAD family member 2
1.5302
0.0100
24 h/12 h
CLDN7
CLDN7-207
Claudin 7
1.8133
3.4290E-06
CLDN7
CLDN7-201
Claudin 7
1.6226
0.0201
CLDN7
CLDN7-202
Claudin 7
1.6204
0.0221
(b) EMT-related genes were significantly downregulated by berberine
Gene_name
Trans_name
Description
Fold_change
_value
12 h/0 h
MMP28
MMP28-201
Matrix metallopeptidase 28
0.5887
0.0398
VIM
VIM-204
Vimentin
0.6486
0.0102
TMEM42
TMEM42-201
Transmembrane protein 42
0.6486
0.0103
24 h/0 h
SMAD3
SMAD3-203
SMAD family member 3
0.3696
0.0338
VIM
VIM-204
Vimentin
0.3877
0.0003
EMP3
EMP3-202
Epithelial membrane protein 3
0.4737
0.0120
VIM
VIM-209
Vimentin
0.5447
0.0011
MMP28
MMP28-201
Matrix metallopeptidase 28
0.5590
0.0101
24 h/12 h
VIM
VIM-204
Vimentin
0.5979
0.0115
VIM
VIM-209
Vimentin
0.6398
0.0036
(a) and (b) The level of transcriptional was significantly upregulated and downregulated BBR treatment related to EMT genes (difference from large to small).
