Oxidative Medicine and Cellular Longevity / 2021 / Article / Tab 1

Research Article

Berberine Suppresses EMT in Liver and Gastric Carcinoma Cells through Combination with TGFβR Regulating TGF-β/Smad Pathway

Table 1

Analyze BBR treatment by transcriptome sequencing assay.
(a) The target genes of BBR related to EMT were significantly upregulated

Gene_nameTrans_nameDescriptionFold_change_value

12 h/0 h
SMAD2SMAD2-205SMAD family member 26.72230.03633
SMAD2SMAD2-207SMAD family member 21.89560.0060
TAB2TAB2-209TGF-beta activated kinase 1 (MAP3K7) binding protein 21.58110.0015
SMAD6SMAD6SMAD family member 61.50900.0000
24 h/0 h
SMAD2SMAD2-205SMAD family member 26.96330.0215
TJAP1(ZO1)TJAP1-201Tight junction associated protein 12.20250.03990
CLDN7CLDN7-201Claudin 71.88720.00161
SMAD6SMAD6SMAD family member 61.55295.1607E-06
JMYJMY-201Junction mediating and regulatory protein, p53 cofactor1.88397.4150E-09
SMAD2SMAD2-207SMAD family member 21.53020.0100
24 h/12 h
CLDN7CLDN7-207Claudin 71.81333.4290E-06
CLDN7CLDN7-201Claudin 71.62260.0201
CLDN7CLDN7-202Claudin 71.62040.0221

(b) EMT-related genes were significantly downregulated by berberine

Gene_nameTrans_nameDescriptionFold_change_value

12 h/0 h
MMP28MMP28-201Matrix metallopeptidase 280.58870.0398
VIMVIM-204Vimentin0.64860.0102
TMEM42TMEM42-201Transmembrane protein 420.64860.0103
24 h/0 h
SMAD3SMAD3-203SMAD family member 30.36960.0338
VIMVIM-204Vimentin0.38770.0003
EMP3EMP3-202Epithelial membrane protein 30.47370.0120
VIMVIM-209Vimentin0.54470.0011
MMP28MMP28-201Matrix metallopeptidase 280.55900.0101
24 h/12 h
VIMVIM-204Vimentin0.59790.0115
VIMVIM-209Vimentin0.63980.0036

(a) and (b) The level of transcriptional was significantly upregulated and downregulated BBR treatment related to EMT genes (difference from large to small).

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