SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression

<div>Observation of SIRT1 expression, cell apoptosis, and autophagy in human-degenerated NP samples. (a) Representative lumbar MRI images showing grade I-II and grade V discs in LVF (left) and IDD (right), respectively. Red arrows indicate the target NP tissue. (b, c) Immunohistochemical staining and western blotting results showing the expression of SIRT1, LC3B, and cleaved caspase 3 in NP tissue from LVF and IDD patients. <span class="nowrap"><svg height="12.7373pt" id="M14" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 106.526 12.7373" width="106.526pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M372 352C427 368 507 407 507 502C507 548 486 592 451 616C420 638 376 650 301 650H44V622C124 616 128 610 128 527V123C128 40 122 34 36 28V0H257C336 0 402 12 453 40C512 72 548 124 548 191C548 291 471 335 372 350V352ZM213 362V558C213 591 216 602 226 608C235 614 258 618 282 618C377 618 415 558 415 490C415 406 369 362 260 362H213ZM213 328H258C380 328 450 283 450 181C450 76 377 35 296 34C231 34 213 53 213 125V328Z"></path></g><g transform="matrix(.013,0,0,-0.013,7.566,0)"><path d="M238 0V26C174 32 166 38 166 104V712C132 700 70 683 18 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NP: nucleus pulposus; LVF: lumbar vertebral fracture; IDD: intervertebral disc degeneration.</div>

Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression 