SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression

<div>SIRT1 overexpression rescued autophagy and reduced cell apoptosis in a rat tail compression model. (a) Appearance and X-ray views of the modified rat tail static compression model. (b) T2-weighted MRI image of the experimental rat tail IVD under high-magnitude compression at 4 weeks in each group. (c) Histomorphological changes in the experimental rat tail IVD under high-magnitude compression. (d) Immunohistochemical staining of cleaved caspase 3, LC3B, and p-P65 expression in experimental rat tail IVD samples from each group. <span class="nowrap"><svg height="12.7373pt" id="M24" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 110.219 12.7373" width="110.219pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M956 650H736V622C814 613 818 602 804 542C789 471 733 275 691 141H689C628 314 570 476 514 641H485C434 478 377 312 323 142H321C277 284 238 419 199 554C184 605 186 613 259 622V650H16V622C77 615 92 606 117 524C172 346 228 170 281 -11H312C363 151 419 312 475 488H477C528 343 597 150 652 -11H683C765 269 826 458 848 522C878 605 887 613 956 622V650Z"></path></g><g transform="matrix(.013,0,0,-0.013,12.466,0)"><path d="M513 0V26C455 31 447 37 447 103V278C447 398 390 449 309 449C255 449 202 412 166 376V712C127 700 67 684 19 677V653C85 647 87 643 87 580V103C87 37 79 31 19 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338C294 338 347 275 347 187C347 112 305 39 221 39C160 39 119 71 97 89C88 97 80 96 71 90C59 80 50 67 49 57C48 45 52 36 66 23C80 9 123 -12 169 -12C221 -11 288 15 342 59C403 109 431 165 431 225C431 308 366 395 238 395C212 395 165 379 127 364L153 550Z"></path></g></svg>.</span> IVD: intervertebral disc; IDD: intervertebral disc degeneration.</div>

Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression 