MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 1

miR-145 expedites repair of SCI in rats. (a) Quantitative analysis for BBB score of neurological function after SCI. (b) HE staining of spinal cord tissue at the injury site (×200). (c) Relative expression level of miR-145 in the rat spinal cord tissue after qRT-PCR detection. (d) miR-145-shuttled adenovirus was injected to rats on the 4th day from the SCI modeling, and the spinal cord tissues were collected 4 weeks later for qRT-PCR determination of miR-145 expression. (e) BBB score to evaluate the neurological function of rats in response miR-145 overexpression. (f) HE staining to assess the normal motor neuron index in all motor neurons (normal+abnormal neurons). (g) Quantitative analysis for number of normal motor neurons in the spinal cord by Nissl staining. Unpaired -test was employed for data comparison between two groups. Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. versus sham-operated rats, ^# versus SCI rats with miR-145 NC (, , ), .

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