MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 2

Overexpression of miR-145 protects PC12 cells from LPS-induced cell damage. (a) qRT-PCR detection of miR-145 expression in PC12 cells after transfection with miR-145 mimic. (b) qRT-PCR detection of mRNA levels of proinflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) in response to LPS treatment and miR-145 mimic. (c) Western blot assay of protein levels of proinflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) in response to LPS treatment and miR-145 mimic. (d) CCK-8 assay to measure cell viability in response to LPS treatment and miR-145 mimic. (e) Flow cytometry to measure cell apoptosis in response to LPS treatment and miR-145 mimic. (f) Western blot assay to measure protein levels of apoptosis-related factors in response to LPS treatment and miR-145 mimic. (g) ELISA to measure proinflammatory cytokine release concentration in a cell culture supernatant in response to LPS treatment and miR-145 mimic. Data were expressed as . Unpaired -test was employed for data comparison between two groups. Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. versus the -LPS group, ^# versus the mimic-NC+LPS group. Cell experiments were repeated three times.

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