MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 3

miR-145 targets KDM6A. (a) Bioinformatic website to predict the binding sites between miR-145 and KDM6A. (b) qRT-PCR detection of the relative expression of KDM6A mRNA in the spinal cord tissue of rats () in response to miR-145 mimic. (c) Dual luciferase reporter assay to validate binding of miR-145 to KDM6A. (d) qRT-PCR to measure miR-145 expression in response to miR-145 mimic and miR-145 inhibitor. (e) qRT-PCR to measure KDM6A mRNA expression in response to miR-145 mimic and miR-145 inhibitor. (f, g) Western blots (f) and statistics (g) of KDM6A protein expression in response to miR-145 mimic and miR-145 inhibitor. Data were expressed as . Independent sample -test was employed for data comparison between two groups. versus the mimic-NC group, ^# versus the inhibitor-NC group (, , ). Cell experiments were repeated three times.

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