MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 4

miR-145 protects PC12 cells from LPS-induced cell damage through KDM6A. (a) qRT-PCR to measure miR-145 expression (top), and Western blot assay to measure KDM6A protein expression (bottom) in response to miR-145 mimic and KDM6A overexpression. (b) CCK-8 assay to measure viability of LPS-treated PC12 cells in response to miR-145 mimic and KDM6A overexpression. (c) Flow cytometry to assess apoptosis of LPS-treated PC12 cells in response to miR-145 mimic and KDM6A overexpression. (d) Western blot assay of protein levels of apoptosis-related factors in response to miR-145 mimic and KDM6A overexpression. (e) ELISA detection of proinflammatory cytokine release in cell culture fluid in response to miR-145 mimic and KDM6A overexpression. Data were expressed as . Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. Statistical analysis in relation to time-based measurements within each group was realized using repeated measures ANOVA, followed by a Bonferroni’s post hoc test. versus the LPS+mimic-NC+oe-NC group, ^# versus the miR-145-mimic+oe-NC group (, , ). Cell experiments were repeated three times.

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