MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 6

miR-145 attenuates NOTCH2 expression through KDM6A to protect PC12 cells from LPS-induced cell damage. (a) qRT-PCR to measure miR-145 expression (top) and Western blot assay to measure KDM6A and NOTCH2 protein expression (bottom) in LPS-induced cells in response to miR-145 mimic and NOTCH2 overexpression. (b) CCK-8 assay to measure cell viability in LPS-induced cells in response to miR-145 mimic and NOTCH2 overexpression. (c) Flow cytometry to measure cell apoptosis in LPS-induced cells in response to miR-145 mimic and NOTCH2 overexpression. (d) Western blot assay to determine protein levels of apoptosis-related factors in LPS-induced cells in response to miR-145 mimic and NOTCH2 overexpression. (e) ELISA to determine concentration of proinflammatory cytokine release in LPS-induced cell culture fluid in response to miR-145 mimic and NOTCH2 overexpression. Data were expressed as . Independent sample -test was employed for data comparison between two groups. Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. Statistical analysis in relation to time-based measurements within each group was realized using repeated measures ANOVA, followed by Bonferroni’s post hoc test. versus the LPS+mimic-NC+oe-NC group, ^# versus the LPS+miR-145-mimic+oe-NC group (, , ). Cell experiments were repeated three times.

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