MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 7

miR-145 confers neuroprotection in rats with SCI by regulating KDM6A in vivo. (a) qRT-PCR detection of the relative expression of miR-145 in the spinal cord tissue of each group of rats. (b) Western blot assay to detect the protein expression of KDM6A, NOTCH2, and Abcb1a in the spinal cord tissue of each group of rats. (c) BBB score to evaluate the neurological function of rats of each group. (d) Quantitative analysis for the normal motor neuron index by HE staining in all motor neurons (normal+abnormal neurons). (e) Quantitative analysis for number of normal motor neurons in spinal cord by Nissl staining. (f) Quantitative analysis for the apoptotic rate of neurons of spinal cord of rats by TUNEL assay. Data were expressed as . Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. versus the sham-operated rats or SCI+miR-NC+oe-NC group, ^# versus the SCI+miR-145+oe-NC group (, , ), .

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