Pharmacological Activation of Nrf2 by Rosolic Acid Attenuates Endoplasmic Reticulum Stress in Endothelial Cells

Figure 8

CRISPR/Cas9-mediated knockout of Nrf2 expression in EA.hy926 cells. The endothelial cells were transfected with Nrf2-CRISPR (a). CAS9-GFP plasmid and GFP+ cells expression were screened using FACS (b). Western blot analysis for Nrf2-expression was performed to confirm successful Nrf2-depletion (c).

(a)

(b)

(c)