Antifungal Treatment Aggravates Sepsis through the Elimination of Intestinal Fungi

Figure 1

Antifungal treatment hastened death of mouse sepsis model. (a) Layout of the experimental setup; Fluc represents fluconazole (b–g). (b) Relative fungal burden in mouse stool was determined by qPCR of fungal 18 s rDNA, -10 per group. (c) Serum alanine aminotransferase (ALT) level at the 14^th day of indicated groups, -7 per group. (d) Survival curve of the cecal ligation and puncture (CLP) model, -9 per group. (e) Survival curve of the MRSA-induced sepsis model at the dose of CFU/mouse, per group. (f) Survival curve of the Salmonella typhimurium- (S.Tyr-) induced sepsis model at the dose of CFU/mouse, per group. (g) Survival curve of the lipopolysaccharide- (LPS-) induced sepsis model, per group. (h) Survival curve of the LPS-induced sepsis model on the water control or amphotericin B treated mice, per group. Data were from one of two (b–e) or four independent experiments (g). Two group comparisons were conducted with a standard two-tailed unpaired Student’s -test (b, c). Mortality was compared by Kaplan-Meier survival curves and analyzed by the log-rank (Mantel-Cox) test (d–h).

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