Broussochalcone A Induces Apoptosis in Human Renal Cancer Cells via ROS Level Elevation and Activation of FOXO3 Signaling Pathway

Figure 1

Antiproliferative effects of BCA against renal cancer cell lines. Dose- and time-dependent effects of BCA (0, 5, 10, 20, and 40 μM) against (a) A498 and (b) ACHN cells after treatment of BCA for 24, 48, and 72 h. The cell viability was detected using the MTT assay. Cell counting assay of (c) A498 and (d) ACHN cells treated with BCA (0, 5, 10, and 20 μM) for 24, 48, and 72 h. Colony formation assay of (e) A498 and (g) ACHN cells treated with BCA (0, 5, 10, and 20 μM) following 2 weeks. (f, h) The error bar represented the standard error. Significant differences between BCA treat and DMSO control groups are indicated as ( means , means , means ).

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