ELK1 Promotes Epithelial-Mesenchymal Transition and the Progression of Lung Adenocarcinoma by Upregulating B7-H3

Figure 1

ELK1 is an important transcriptional regulator of B7-H3. (a) Heat map of expression of transcription factors of B7-H3 in LUAD samples and normal samples (left); analysis of the correlation between the expressions of ELK1 and B7-H3 in pan-cancer (right). (b) Knockdown of ELK1 inhibited the expression levels of B7-H3 mRNA and protein in A549 or H1299 cell lines. (c) Overexpression of ELK1 promoted the expression of B7-H3 mRNA and protein in H460 or H1975 cell lines. (d) Motif of promoter sequence of B7-H3 can bind to ELK1. (e) Schematic diagram of the B7-H3 promoter, including the predicted ELK1-binding regions (left) (the wild-type (WT) or mutant (MUT)) and binding site of B7-H3 which was synthesized for the luciferase reporter assay in 293 T cell lines (right). (f) ChIP analysis was used to determine the binding of ELK1 to the B7-H3 promoter, IgG as a negative control. , , and .

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