ULK1 Suppresses Osteoclast Differentiation and Bone Resorption via Inhibiting Syk-JNK through DOK3

Figure 1

ULK1 is associated with osteoclast differentiation and bone loss. (a) Heat map of differentially expressed mRNA of ULK1 in osteoclasts (OC) and bone marrow macrophage (BMM) from dataset GSE54779 in GEO. (b) Statistical analysis of ULK1 expression between BMM and OC in a heat map. (c) Unc-51-like autophagy activating kinase 1 (ULK1) expression during osteoclast differentiation of RAW264.7 cells. (d) ULK1 expression during osteoclast differentiation of mouse BMM. (e, f) ULK1 mRNA (e) or protein (f) expression in BMM from the sham and OVX groups. (g) Immunofluorescence analysis of ULK1 expression (green) in BMM between the sham and OVX groups. BMM was stained with integrin alpha-M (CD11b) (red). Nucleus was stained with DAPI (blue). The red arrowhead points to BMM. The white line showed the boundary between bone and bone marrow cavity (scale bar, 50 μm). (h) Immunofluorescence analysis of ULK1 expression (green) in OC between sham and OVX mice. OC was stained with cathepsin K (CTSK) (red). Nucleus was stained with DAPI (blue). The yellow line points to OC. The white line showed the boundary between bone and bone marrow cavity (scale bar, 50 μm). (i) Quantification of ULK1 expression in BMM in (g). (j) Quantification of ULK1 expression in OC in (h). (k) TRAP staining (left) and immunohistochemistry (right) of ULK1 in femur sections from the OVX and sham groups. The yellow arrowhead points to OC (scale bar, 250 μm). All data are ; , , .

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