ULK1 Suppresses Osteoclast Differentiation and Bone Resorption via Inhibiting Syk-JNK through DOK3

Figure 3

JNK signalling mediates the effect of ULK1. (a, b) Western blotting (a) and quantitative analysis (b) of c-Jun N-terminal kinase (JNK) signalling in RAW264.7 cells overexpressing ULK1 treated with 50 ng/ml of RANKL for 0–60 minutes. (c, d) Western blotting (c) and quantitative analysis (d) of JNK signalling in RAW264.7 cells with ULK1 knockdown treated with 50 ng/ml RANKL for 0–60 minutes. (e, f) TRAP staining (e) and quantification (f) in Si-NC, JNK-IN-8, and Si-ULK1 with JNK-IN-8 OC (scale bar, 100 μm). (g) OC-specific gene expression in Si-NC, JNK-IN-8, and Si-ULK1 with JNK-IN-8 BMM. (h, i) Western blot (h) and quantitative analysis (i) of p-Syk and total spleen tyrosine kinase (Syk) levels in Si-NC and Si-ULK1 RAW264.7 cells. (j, k) Western blot (j) and quantitative analysis (k) of p-JNK and total JNK levels in Si-NC and Si-ULK1 cells treated with or without PRT062607. (l, m) TRAP staining (l) and quantification (m) in BMM of Si-NC, Si-ULK1, and Si-ULK1 treated with PRT062607 (scale bar, 100 μm). (n) The expression of OC-specific genes in BMM of Si-NC, Si-ULK1, and Si-ULK1 treated with PRT062607. All data are ; ns . , , .

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