Research Article
Calorie Restriction Protects against Contrast-Induced Nephropathy via SIRT1/GPX4 Activation
Figure 2
Effect of CR on apoptosis of renal outer medulla in SD rats with CIN. SD rats were pretreated with CR for 4 weeks. CIN was induced by the intravenous injection of the CM iopromide (1.8 g/kg). The kidney samples were collected 24 h after CM injection. (a1) HE staining of renal outer medulla ( μm). (a2) Pathological scores. (b1) TUNEL staining of renal outer medulla ( μm) and (b2) TUNEL-positive cells counted in 10 high-power (400×) fields. (c) Western blot analysis of cleaved caspase 3 (a marker of apoptosis), total caspase-3 expression, and cleaved caspase 3/total caspase 3 ratio. The values are presented as fold-change of control group. (d) Caspase-3 activity detected by ELISA. The values are fold change of the control group; each control sample is given a value of 1. The values are presented as (, vs. control, # vs. CM alone).
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