Calorie Restriction Protects against Contrast-Induced Nephropathy via SIRT1/GPX4 Activation

Figure 3

Role of SIRT1 in the renal protective effect of CR on CIN. SD rats were pretreated with CR for 4 weeks. CIN was induced by the intravenous injection of the CM iopromide (1.8 g/kg). The SIRT1 inhibitor EX527 (500 mg/kg) was injected intravenously before establishment of CIN. The kidney samples were collected 24 h after CM injection. (a) Western blot analysis of SIRT1 expression in kidney tissues. Values are presented with fold change of the control group; each control sample is given a value of 1. (b1) HE staining of renal outer medulla ( μm). (b2) Pathological scores are shown. Serum Cr (c) and BUN (d) levels were measured in different groups, 24 h after CM injection. The values are presented as (, vs. control, ^# vs. CM alone, ^& vs. CM+CR).

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