Exosomes Released from CaSR-Stimulated PMNs Reduce Ischaemia/Reperfusion Injury

Figure 5

The exosomes from CaSR-stimulated PMNs significantly inhibited cardiomyocyte apoptosis after H/R injury. (a) In cardiomyocytes, the expression of p-AKT, Bcl-xl, and Bax after coculture with exosomes released from PMNs was detected by western blot. (b) The cardiomyocyte apoptosis rate was detected. The results are representative of three experiments, and the apoptosis rate under different conditions was quantified by densitometry. Q1: necrotic cells. Q2: late apoptotic cells. Q3: living cells. Q4: early apoptotic cells. (c) Exosomes from CaSR-stimulated PMNs decreased Nox2 expression and ROS production from cardiomyocytes during H/R injury. (d) Exosomes from CaSR-stimulated PMNs decreased ROS production in cardiomyocytes during H/R injury. Data are shown as the ; ^# vs. control group, vs. H/R group, and ^△ vs. CIN group. Data are representative of three independent experiments.

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