MED1 Deficiency in Macrophages Accelerates Intimal Hyperplasia via ROS Generation and Inflammation

Figure 2

Macrophage MED1 deficiency promotes arterial injury-induced vascular inflammation. (a) Expression of proinflammatory genes in injured arteries from littermate (MED1^fl/fl) or macrophage MED1 knockout (MED1^ΔMac) mice 7 d after ligation. Twelve mice were used in each group, and samples from every four mice were pooled. (b, c) ELISA analysis of TNFα levels in plasma collected from 7-day or 21-day common carotid artery injured mice (). (d) Representative immunohistochemistry staining and quantification of TNFα levels in artery cross sections from 21-day carotid artery injured mice (). (e) Representative immunohistochemistry staining and quantification of Mac-3 (macrophages) in artery cross sections from 21-day carotid artery injured mice (). The data are expressed as the . ; . PDGFA: platelet-derived growth factor, alpha; PDGCB: platelet-derived growth factor, B polypeptide.

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