MED1 Deficiency in Macrophages Accelerates Intimal Hyperplasia via ROS Generation and Inflammation

Figure 3

Macrophage MED1 deficiency promotes migration and adhesion of macrophages. (a) qRT-PCR analysis for MCP-1, CCR2, and CCR4 levels in peritoneal macrophages from MED1^fl/fl or MED1^ΔMac mice treated with LPS (50 ng/mL) for 6 h. (b) Representative images (b, left) of peritoneal macrophages subjected to Transwell assays. MCP-1 (20 ng/mL) was used to induce migration for 24 h. Bar chart (b, right) showing the fold change of migrated macrophages (). (c) qRT-PCR analysis of ICAM1 and VCAM1 levels in peritoneal macrophages from MED1^fl/fl or MED1^ΔMac mice treated with LPS (50 ng/mL) for 6 h. (d) qRT-PCR analysis of M-CSF and CSF1R levels in peritoneal macrophages from MED1^fl/fl or MED1^ΔMac mice treated with LPS (50 ng/mL) for 6 h. The data are expressed as the (–8). ; .

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