Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

Figure 3

Antioxidant activity assays and cell viability assay. The antioxidant assays were performed using the Commiphora leptophloeos extract concentration at 50, 100, and 250 μg/mL. (a) Reducing power assay. The -axis corresponds to the different extracts and concentrations used. The -axis corresponds to the activity percentage, based on the standard curve using ascorbic acid as a standard. (b) DPPH assay. The -axis corresponds to the different extracts and concentration. The -axis corresponds to the DPPH percentage scavenging. (c) Superoxide radical scavenging assay. The -axis corresponds to the different C. leptophloeos extracts. The -axis corresponds to the percentage scavenging. (d) Cell viability of the NHI/3T3 cell line after 24 h of ethanol extract (EE) treatment. The -axis corresponds to EE concentration at 100 μg/mL, 250 μg/mL, and 500 μg/mL. The -axis corresponds to the percentage of cell viability via MTT reduction. (e) Cell viability of the B16F10 tumour cell line after 24 h of EE treatment. The -axis corresponds to EE concentration at 100 μg/mL, 250 μg/mL, and 500 μg/mL. The -axis corresponds to the cell viability percentage via MTT reduction. EH: hexane extract; EC: chloroform extract; EE: ethanol extract; EM: methanol extract; EAR: residual aqueous extract; EA: aqueous extract. All assays (a–e) were done in triplicate for each extract, and the data obtained were analysed using ANOVA and Tukey’s test (). Different letters (a, b, c, d, e, and f) correspond to significant differences.

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