Antioxidant Activities of Commiphora leptophloeos (Mart.) J. B. Gillett) (Burseraceae) Leaf Extracts Using In Vitro and In Vivo Assays

Figure 5

Ethanol extract (EE) antioxidant effect on Caenorhabditis elegans model. (a) Levels of reactive oxygen species (ROS) in N2 wild-type animals treated with the C. leptophloeos EE for 48 h. (b) Survival assay of N2 wild-type animals under oxidative stress conditions with t-BOOH. Animals were treated with 0, 100, 500, or 1000 μg/mL of EE at L1 to L4. The -axis corresponds to the hours observed (3 to 30 h), and the -axis, to the survival fraction. Three independent experiments were performed, with three replicates per treatment. The EE treatment was compared to the control by the Log-rank test (). (c) sod-3::GFP expression in C. elegans after EE treatment 0, 100, 500, or 1000 μg/mL. The images were taken using the Olympus BX51 microscope. (d) sod-3::GFP expression was quantified. The -axis corresponds to different concentrations of C. leptophloeos EE. The -axis corresponds to GFP expression quantification. For all assays, three independent experiments were performed, with three replicates per treatment. Different letters correspond to significant differences. Statistical analysis was performed using ANOVA and Tukey test ().

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