Research Article
Novel Synthetic Coumarin-Chalcone Derivative (E)-3-(3-(4-(Dimethylamino)Phenyl)Acryloyl)-4-Hydroxy-2H-Chromen-2-One Activates CREB-Mediated Neuroprotection in Aβ and Tau Cell Models of Alzheimer’s Disease
Figure 5
LM-021-mediated kinase activation in CRE-GFP reporter cells. (a) Fluorescence analysis of CRE reporter cells without and with kinase inhibitor, Ca2+ ionophore, and/or forskolin or LM-021 treatment. GFP fluorescence was assessed by flow cytometry (). To normalize, the GFP fluorescence level in untreated cells was set as 100%. (b) p-CREB and GFP levels analysed by immunoblot using GAPDH as a loading control (). To normalize, the protein expression level in untreated cells was set as 100%. values: compound treated vs. untreated cells (, , and ), or kinase inhibitor-treated vs. untreated cells (&&&: ) (one-way ANOVA with a post hoc Tukey test).
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