Echinacoside Upregulates Sirt1 to Suppress Endoplasmic Reticulum Stress and Inhibit Extracellular Matrix Degradation In Vitro and Ameliorates Osteoarthritis In Vivo

Figure 4

ECH reduced TBHP-stimulated chondrocyte apoptosis by preventing ER stress. (a) The mRNA expression levels of ER stress-related biomarker proteins in each group were detected by using real-time PCR analysis. (b, c) ROS in mouse chondrocytes were assessed with the ROS Assay Kit. (d–j) The protein expression levels of BAX and PERK-eIF2α-ATF4-CHOP pathway-related biomarkers were assayed by western blot analysis. (k, l) The representative CHOP was detected by immunofluorescence combined with DAPI staining for nuclei (bar: 50 μm). All values represent (). , . TBHP: tert-Butyl hydroperoxide; ECH: Echinacoside; TG: thapsigargin; ROS: reactive oxygen species.

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