Echinacoside Upregulates Sirt1 to Suppress Endoplasmic Reticulum Stress and Inhibit Extracellular Matrix Degradation In Vitro and Ameliorates Osteoarthritis In Vivo

Figure 6

Sirt1 silencing abrogated the ECH-mediated protection against TBHP-induced OS. (a) After knocking down Sirt1 with siRNA, real-time PCR was used to test the knockdown effect. (b) The mRNA expression levels of BAX, GRP78, ATF4, and CHOP in each group were examined by real-time PCR analysis. (c–h) The protein expression levels of Sirt1, GRP78, ATF4, CHOP, and BAX were assayed by western blot analysis. All values represent (). , . TBHP: tert-Butyl hydroperoxide; ECH: Echinacoside.

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