Echinacoside Upregulates Sirt1 to Suppress Endoplasmic Reticulum Stress and Inhibit Extracellular Matrix Degradation In Vitro and Ameliorates Osteoarthritis In Vivo

Figure 7

ECH prevents TBHP-stimulated ECM destruction in chondrocytes. (a) Real-time PCR analysis was used to examine the mRNA levels of Aggrecan, ADAMTS5, MMP13, and Collagen II in each group. (b–f) The protein expression levels of Aggrecan, ADAMTS5, MMP13, and Collagen II were assayed by western blot analysis. (g–j) Immunofluorescence staining of Collagen II and MMP13 and quantitation of the number of chondrocytes positive for Collagen II and MMP13 in different groups. Markedly increased green bright puncta indicated the upregulation of Collagen II protein expression (bar: 20 μm). All values represent (). , . TBHP: tert-Butyl hydroperoxide; ECH: Echinacoside.

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