Echinacoside Upregulates Sirt1 to Suppress Endoplasmic Reticulum Stress and Inhibit Extracellular Matrix Degradation In Vitro and Ameliorates Osteoarthritis In Vivo

Figure 8

Sirt1 silencing abrogated ECH-mediated protection of ECM under induced OS. (a–e) The protein expression levels of Aggrecan, ADAMTS5, MMP13, and Collagen II were assayed by western blot analysis. (f–i) Immunofluorescence staining of MMP13 and Collagen II and quantitation of the number of chondrocytes positive for MMP13 and Collagen II in different groups. Markedly increased green bright puncta indicated the upregulation of MMP13 and Collagen II protein expression (bar: 20 μm). All values represent (). , . TBHP: tert-Butyl hydroperoxide; ECH: Echinacoside.

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