Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction
Inflammatory cytokines promoted the activation of tRAS in human nucleus pulposus cells in vitro. (a, b) Secretion amount of Ang II in supernatant of human NP cells induced by IL-1β or TNF α was quantified by the ELISA assay (). (c, d) Western blot revealed the relative protein expression of tRAS components (ACE and AT1) in human NP cells induced by IL-1β or TNF α (). (e, f) RT-qPCR quantified the relative mRNA expression tRAS components (ACE and AT1) in human NP cells induced by IL-1β or TNF α in a time-change manner ().,,,.
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