Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction

Figure 3

Angiotensin II triggered human NP cells senescence in a dose-dependent manner. (a) The SA-β-gal staining of human NP cells induced by Ang II (). (b) Quantitative results of SA-β-gal-positive NP cells in different groups (). (c) Immunofluorescence results for the expression of γH2AX in human NP cells treated by Ang II (). . (d) The expression of γH2AX was quantified as averaged fluorescent voxels/cell (). (e) The protein expression of senescence-related markers, p53, and Klotho in human NP cells induced by Ang II (). , , , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)