FAM134B-Mediated ER-phagy Upregulation Attenuates AGEs-Induced Apoptosis and Senescence in Human Nucleus Pulposus Cells

Figure 4

ROS inhibition partially attenuated AGEs-induced ER-phagy activation in human NP cells. The human NP cells were treated with 200 μg/mL AGEs and 10 μM NAC for 36 h. (a–d) ER-phagy-associated protein levels of FAM134B, LC3, and p62 were detected by western blot assay, and the relative band density was quantified. (e) Representative fluorescent images of FAM134B and LC3 were evaluated, and cell nuclei were stained with DAPI. Scale bar: 50 μm. (f) ER and lysosome colocalization was labeled with ER-tracker and Lyso-tracker staining. Data are represented as . , .

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