TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization

Figure 4

Myeloid-specific TL1A overexpression promotes the recruitment of macrophages into the liver in mice with NASH. (a1) Relative mRNA levels of F4/80 in mice of HFHF model were determined by quantitative real-time PCR. (a2) Relative mRNA levels of F4/80 in mice of MCD model were determined by quantitative real-time PCR. (b1, b3) F4/80 protein in mice of HFHF model was detected by immunofluorescence staining (400x). (b2, b4) F4/80 protein in mice of MCD model was detected by immunofluorescence staining (400x). (c1) The expression of CCL2 and CXCL1 in liver tissues of mice with fed HFHF diets was detected by quantitative real-time PCR and ELISA, respectively. (c2) The expression of CCL2 and CXCL1 in liver tissues of mice with fed MCD diets was detected by quantitative real-time PCR and ELISA, respectively. (c3) The expression of CCR2 and CXCR2 in liver tissues of mice with fed HFHF and MCD diets was detected by quantitative real-time PCR. (c4) The expression of CCR2 and CXCR2 in liver tissues of mice with fed HFHF and MCD diets was detected by Western blotting. Data are expressed as , , , .

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