TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization

<div>Macrophages with TL1A overexpression induce lipid accumulation, proinflammatory cytokine production, and apoptosis in hepatocytes <i>in vitro</i>. (a1, a2) Supernatant ALT and AST in hepatocytes were measured by automatic biochemical analyzer. (b1–b3) Lipid accumulation, intercellular cholesterol, and triglyceride content in primary hepatocytes were analyzed. The expression of TNF-<i>α</i>, IL-1<i>β</i>, and IL-6 in hepatocytes (c1) and these supernatant (c2) was detected by quantitative real-time PCR and ELISA, respectively. (d1) The percentage of apoptotic hepatocytes was measured by TUNEL. (d2) The expression of cleaved-caspase3 and cleaved-PARP in hepatocytes was detected by Western blotting. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization 