Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy

Figure 4

rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac p-AMPK, CPT-1, PGC1-ɑ, PPAR-α, CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR-α, CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μm. The arrows indicate positively stained cells. per group. vs. db/db+rhGH group, vs. db/db+rhGH group, ^& vs. db/db group, and ^# vs. db/db group.

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