Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy

Figure 5

rhGH decreased oxidative stress in db/db mice. (a) ROS-stained (red fluorescence) and DAPI-stained (blue fluorescence) photomicrographs. Scale bar: 100 μm, per group. vs. db/db+rhGH group and ^# vs. db/db group. (b) Cardiac MDA and GSH activities were quantified using commercial assay kits. per group. vs. db/db+rhGH group and ^# vs. db/db group. (c) Cardiac NRF2, HO-1, SOD, NOX4, and CAT levels were measured by western blotting. (d) Relative protein expression was quantified. per group. vs. db/db+rhGH group, ^& vs. db/db group, and ^# vs. db/db group.

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