Changes in Hepcidin Levels in an Animal Model of Anemia of Chronic Inflammation: Mechanistic Insights Related to Iron Supplementation and Hepcidin Regulation

Figure 11

Western blot analysis was performed using HepG2 cells transfected with control small interfering RNA (siRNA) or siRNA against signal transducer and activator of transcription-3 (STAT-3) and sma mothers against decapentaplegic 1/5 (SMAD1/5) to investigate hepcidin expression after iron treatment (2.5 mg/mL). The relative protein levels were quantified using densitometry (b–d and f–i). siCONTROL: control siRNA; siSTAT-3: siRNA against STAT-3; P-STAT-3: phosphorylated STAT-3; siSMAD1/5: siRNA against SMAD1/5; P-SMAD1/5: phosphorylated SMAD1/5; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; C: control group; I: iron-treated group. compared with the control group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)