Oxidative Medicine and Cellular Longevity / 2021 / Article / Fig 3

Research Article

Clostridium butyricum Protects IPEC-J2 Cells from ETEC K88-Induced Oxidative Damage by Activating the Nrf2/ARE Signaling Pathway

Figure 3

Effect of CB on Nrf2 signaling in ETEC K88-infected IPEC-J2 cells. (a–e) Real-time PCR was used to detect the relative expression level of SOD1, SOD2, GPX1, GPX2, and Nrf2, calculated against the housekeeping gene GAPDH. (f) The Nrf2 protein levels after ETEC infected were measured by Western blot analysis. The results were of three independent preparations. Value columns with different lowercase letters mean , while with different capital letters mean .
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