The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue

Figure 5

Effect of an acute exercise session on biomarkers of oxidative damage in the retroperitoneal WAT of rats. (a) Levels of reduced thiol content. Total sulfhydryl groups were measured in homogenates of retroperitoneal WAT by spectrophotometry. DTNB: 5,5-dithiobis-(2-nitrobenzoic acid) (/group). (b) Levels of lipid peroxidation, representative result of a membrane incubated with anti-4-HNE and Ponceau Rouge. Data were normalized to Ponceau Rouge and expressed as relative to control. 4-HNE: 4-hydroxynonenal (/group). (c) Levels of carbonylated proteins, representative result of a membrane incubated with anti-DNPH and anti-β-actin using the OxyBlot® Kit. Data were normalized to β-actin and expressed as relative to control. DNPH: 2,4-dinitrophenylhydrazine. CTRL: control; 0 h; euthanasia immediately after exercise; 30 min: euthanasia 30 minutes after exercise; 1 h: euthanasia 1 hour after exercise; 2 h: euthanasia 2 hours after exercise. The data were expressed as the (/group). ; , ; .

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