Protective Effect of Mitochondrial ND2 C5178A Gene Mutation on Cell and Mitochondrial Functions

Figure 3

Proliferation and apoptosis of mutant and control cell lines. (a) Caspase 3/7 activity: Caspase 3/7 is a specific molecular marker of apoptosis. The expression level of Caspase 3/7 in the mutant group was significantly lower than that in the control group. (b) The transcription of proliferation and apoptosis genes: the mutant group showed increased Bcl-2 gene transcription compared with the control group. But there was no significant difference between Bax and Cyclin D1 gene transcription. (c) The protein expression of proliferation and apoptosis genes: the mutant cells showed increased Bcl-2 protein level. (d) Apoptosis assay: the apoptosis rate was detected by the FITC Annexin V Apoptosis Detection Kit. The living cell ratio of the mutant group was higher than that of the control group. The early apoptosis and late apoptosis in the mutant group are significantly decreased than those in the control group. ().

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