Research Article
p53 Inhibition Protects against Neuronal Ischemia/Reperfusion Injury by the p53/PRAS40/mTOR Pathway
Figure 6
PRAS40 knockdown inhibited the activities of the mTOR pathway. As shown by immunostaining (a, Ć400) and western blotting (b, bands; c, quantification), in primary cultured p53-/- mouse neurons subjected to OGD, the expression of p-mTOR and p-S6K was distinctly reduced after pretransfection with PRAS40 shRNA compared with that in neurons pretransfected with or without scramble shRNA. vs. the p53-/-+scramble shRNA group. ā21/group in at least 3 independent experiments.
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