Ginsenoside Rg5 Inhibits Human Osteosarcoma Cell Proliferation and Induces Cell Apoptosis through PI3K/Akt/mTORC1-Related LC3 Autophagy Pathway

Figure 2

Rg5 inhibited MG-63 cells via caspase-3 activity that was related to the LC3-mediated autophagy pathway. MG-63 cells were treated with 160 nM of Rg5, and total proteins were extracted. The expression levels related proteins were detected by western blotting, qRT-PCR, or FACS. (a) Representative image of proteins detected by western blotting. The ratio of LC3-II/LC3-I (b) and cleaved caspase-3/caspase-3 (c) in MG-63 cell. (d) The relative gene expression of caspase-3. (e, f) The MFI of LC3 detected by FACS. The data are shown as (, vs. control).

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