Ginsenoside Rg5 Inhibits Human Osteosarcoma Cell Proliferation and Induces Cell Apoptosis through PI3K/Akt/mTORC1-Related LC3 Autophagy Pathway

Figure 3

Inhibition of autophagy reduced Rg5-induced caspase-3 activity and cell apoptosis. 5 mM 3-MA combining or alone with 160 nM Rg5 was used to treat MG-63 cells for 12 h and for caspase-3 and LC3 autophagy-related protein detection by western blotting or FACS. (a) Representative image of proteins detected by western blotting. (b) The ratio of LC3-II/LC3-I in MG-63 cells. (c) The ratio of cleaved caspase-3/caspase-3 in MG-63 cells. (d) The MFI of LC3 in MG-63 cells detected by FACS. (e, f) MG-63 cell apoptosis detected by FACS. The data are shown as (, ^ns, ^#, 3-MA treatment comparing with control; , vs. control).

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