Ginsenoside Rg5 Inhibits Human Osteosarcoma Cell Proliferation and Induces Cell Apoptosis through PI3K/Akt/mTORC1-Related LC3 Autophagy Pathway

Figure 5

The PI3K/AKT/mTORC1 pathway is involved in Rg5-induced human osteosarcoma cell autophagy activation. (a) Representative image of proteins detected by western blotting at 12 h and 24 h after the treatment. (b) Phosphorylated PI3K, Akt, and TORC1 in MG-63 cells. (c) The ratio of LC3/β-actin in MG-63 cells. (d) The ratio of cleaved caspase-3/caspase-3 in MG-63 cells. (e, f) Phosphorylated PI3K, Akt, and TORC1 in MG-63 treated with IGF-1 were detected by WB at 24 h after the treatment. (g) The ratio of LC3/β-actin in MG-63 cells. (h) The ratio of cleaved caspase-3/caspase-3 in MG-63 cells. The data are shown as (, ^# IGF treatment comparing with control; ^ns, , vs. control).

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