Research Article

Therapeutic Role of miR-30a in Lipoteichoic Acid-Induced Endometritis via Targeting the MyD88/Nox2/ROS Signaling

Figure 6

Overexpressing miR-30a prevents LTA-induced NF-κB activation by inhibiting the MyD88/Nox2/ROS axis. (a) BEND cells were cotransfected with miR-30a mimic and pcDNA3.1-MyD88 for 24 h, followed by 12 h of exposure to LTA. Then, the levels of MyD88, Nox2, p-p65, and p65 were measured by Western blot. (b) The levels of ROS were detected using the fluorescent probe DCFH-DA. . (c) Nuclear translocation of NF-κB p65 was assessed by immunofluorescence staining. . Data are presented as . , .
(a)
(b)
(c)