The Antitriple Negative Breast cancer Efficacy of Spatholobus suberectus Dunn on ROS-Induced Noncanonical Inflammasome Pyroptotic Pathway

Figure 9

GSH blocked the efficacy of SSP and the pyroptotic signaling pathways in TNBC cells. (a) Representative result for cell viability detection of 4T1 cells after different treatments, . Data are shown as (). (b) Representative result of LDH release assay in 4T1 cells after different treatments, . Data are shown as (). (b) Representative phase-contrast microscopy of 4T1 cells after different treatments. (c) Representative western blot assay for the detection of pyroptotic signaling pathways in GSH rescue experiment. BT-549 cells were pretreated with or without GSH (2 mM) for 2 h, followed by SSP (100 μg/ml) or vehicle treatment for 24 h, respectively, as specifically indicated.

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