The Antitriple Negative Breast cancer Efficacy of Spatholobus suberectus Dunn on ROS-Induced Noncanonical Inflammasome Pyroptotic Pathway

Table 1

Mortality and clinical signs of acute toxicity of SSP.

Animals	Dose of SSP (g/kg b.w. p.o)	Mortality latency (h)	Toxic symptoms	LD50 (g/kg b.w. p.o.)
BALB/c mice	0	—	None	10
	2.0	—	None
	4.0	—	None
	8.0	—	None
	10.0	<1 h	Anorexia, hypoactivity
Sprague-Dawley rats	0	—	None	>10
	2.0	—	None
	4.0	—	None
	8.0	—	None
	10.0	—	None

Animals were divided into respective 5 groups and 6 animals (3 males and 3 females) each. Each group receiving a single oral dose of 2, 4, 8, and 10 g/kg body weight of SSP extract, while the control group was administrated with distilled water. The general behavior, signs of toxicity and/or death, and the latency of death were observed continuously for 1 h after the oral administration and then intermittently for 4 h and thereafter for 24 h. The LD₅₀ value was determined according to the method described by Kharchoufa et al. [45].