Research Article
The Synergistic Reducing Drug Resistance Effect of Cisplatin and Ursolic Acid on Osteosarcoma through a Multistep Mechanism Involving Ferritinophagy
Figure 4
Iron accumulation in OS cells treated with the combination treatment of UA and Cis. Intracellular Fe2+ levels were measured after treatment with UA and/or Cis as indicated (a, d) (, scale bar: 25 μm). The expression quantity of TFR was assessed by immunofluorescence staining of HOS cells after the indicated treatment (b, e) (, scale bar: 50 μm). The expression of iron metabolism-related proteins in UA- and/or Cis-treated HOS cells was determined by western blotting (c) (). The data are presented as the . vs. the control group.
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