Research Article
Protective Effects of Topical Application of Nitrite on Testicular Ischemia-Reperfusion Injury in Rats
Figure 1
Effects of topically injected nitrite and nitrate on 3-nitrotyrosine (3-NT) and dihydroethidium (DHE) staining in the ipsilateral testis (×200). (a–d) Immunohistochemical staining of 3-NT in the left ischemic testes of groups A, B, C, and G. (e) The number of 3-NT-positive cells per seminiferous tubule in all groups. (f–i) DHE staining of in the left ischemic testes of groups A, B, C, and G. (j) The number of DHE-stained positive cells per seminiferous tubule in all groups. vs. group A, # vs. group B. Groups: A: control, B: ischemia/reperfusion (IR) injury, C: IR+0.12 nmol/g nitrite, D: IR+1.2 nmol/g nitrite, E: IR+12 nmol/g nitrite, F: IR+0.12 nmol/g nitrite+0.01 μmol/g C-PTIO, and G: IR+0.12 nmol/g nitrate.
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