Role of SIRT1 in Hepatic Encephalopathy: In Vivo and In Vitro Studies Focusing on the NLRP3 Inflammasome

Figure 10

High levels of ammonia increased the ROS levels and induced the apoptosis in HT22 neuronal cells. (a) The cell viability of HT22 cells at different concentrations NH₄Cl (2.5, 5, 10, 20, 40, 80, 160, and 320 mM) was detected by CCK-8 assay; (b) the apoptosis rates were determined by flow cytometric analysis; (c) the morphological changes of HT22 cells; (d) cleaved caspase-3 was analyzed by western blotting; (e) the quantitative blots of cleaved caspase-3; (f) cells were treated with NH₄Cl (0, 5, 10, and 20 mM); then, the ROS levels were measured by DCF fluorescence with flow cytometry; (g) the mean DCF-intensity in each group. , compared with the control group. per group. The data represent the .

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