Role of SIRT1 in Hepatic Encephalopathy: In Vivo and In Vitro Studies Focusing on the NLRP3 Inflammasome

Figure 5

CAY10602 alleviated the activation of NLRP3 inflammasome in BV2 cells. (a) The cell viability of BV2 cells at different concentrations CAY10602. (b) BV2 cells exposed to CAY10602 (1, 4, 8, and 10 μM) for 8 h; then, the protein expression of SIRT1 was measured by western blotting. (c) The quantitative blots of SIRT1. (d) Cells were exposed to normal saline, 10 μg/ml LPS+4 mM ATP, 10 μg/ml LPS+4 mM ATP+10 μM CAY10602, and 10 μM CAY10602. The protein expression of SIRT1, NLRP3, ASC, and IL-1β was detected by western blotting. (e) The quantitative blots of SIRT1. (f) The quantitative blots of NLRP3, ASC, and IL-1β. , compared with the control group; ^#, compared with the control+10 μg/ml LPS+4 mM ATP group. per group. The data represent the .

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